Product Info Summary
SKU: | A04924 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF |
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Product info
Product Name
Anti-LILRB4/Gp49 Antibody
View all ILT3/CD85k/LILRB4 Antibodies
SKU/Catalog Number
A04924
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-LILRB4/Gp49 Antibody (Catalog # A04924). Tested in IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LILRB4/Gp49 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04924)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human LILRB4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04924 is reactive to LILRB4 in Human
Applications
A04924 is guaranteed for IF Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
49.356kDa
Background of ILT3/CD85k/LILRB4
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Arm J.P., J. Immunol. 159:2342-2349(1997).
Borges L., J. Immunol. 159:5192-5196(1997).
Liu W.R., Immunogenetics 51:659-669(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of A549 cells, using LILRB4 antibody A04924.
Protein Target Info & Infographic
Gene/Protein Information For LILRB4 (Source: Uniprot.org, NCBI)
Gene Name
LILRB4
Full Name
Leukocyte immunoglobulin-like receptor subfamily B member 4
Weight
49.356kDa
Alternative Names
CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4 LILRB4 CD85K, ILT-3, ILT3, LIR-5, LIR5 leukocyte immunoglobulin like receptor B4 leukocyte immunoglobulin-like receptor subfamily B member 4|CD85 antigen-like family member K|immunoglobulin-like transcript 3|leucocyte Ig-like receptor B4|leukocyte immunoglobulin-like receptor 5|leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4|monocyte inhibitory receptor HM18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LILRB4, check out the LILRB4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LILRB4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LILRB4/Gp49 Antibody (A04924)
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No publications found for A04924
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-LILRB4/Gp49 Antibody
Question
I was wanting to use your anti-LILRB4/Gp49 antibody for IF for human monocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human monocyte identification?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can see on the product datasheet, A04924 anti-LILRB4/Gp49 antibody has been validated for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human monocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-07
Question
My question regarding product A04924, anti-LILRB4/Gp49 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04924 anti-LILRB4/Gp49 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-10
Question
Do you have a BSA free version of anti-LILRB4/Gp49 antibody A04924 available?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LILRB4/Gp49 antibody A04924 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-11
Question
We are currently using anti-LILRB4/Gp49 antibody A04924 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The anti-LILRB4/Gp49 antibody (A04924) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-09
Question
Is this A04924 anti-LILRB4/Gp49 antibody reactive to the isotypes of LILRB4?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
The immunogen of A04924 anti-LILRB4/Gp49 antibody is Synthesized peptide derived from internal of human LILRB4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte using anti-LILRB4/Gp49 antibody A04924. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-11
Question
Here is the WB image, lot number and protocol we used for monocyte using anti-LILRB4/Gp49 antibody A04924. Please let me know if you require anything else.
E. Moore
Verified customer
Asked: 2018-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-23
Question
Does anti-LILRB4/Gp49 antibody A04924 work on goat IF with placenta?
Verified Customer
Verified customer
Asked: 2018-06-11
Answer
Our lab technicians have not validated anti-LILRB4/Gp49 antibody A04924 on goat. You can run a BLAST between goat and the immunogen sequence of anti-LILRB4/Gp49 antibody A04924 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat placenta in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-11
Question
Is a blocking peptide available for product anti-LILRB4/Gp49 antibody (A04924)?
N. Roberts
Verified customer
Asked: 2018-06-01
Answer
We do provide the blocking peptide for product anti-LILRB4/Gp49 antibody (A04924). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-01
Question
Does A04924 anti-LILRB4/Gp49 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
As indicated on the product datasheet, A04924 anti-LILRB4/Gp49 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-24
Question
I see that the anti-LILRB4/Gp49 antibody A04924 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-04
Question
Will anti-LILRB4/Gp49 antibody A04924 work for IF with monocyte?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
According to the expression profile of monocyte, LILRB4 is highly expressed in monocyte. So, it is likely that anti-LILRB4/Gp49 antibody A04924 will work for IF with monocyte.
Boster Scientific Support
Answered: 2017-06-21
Question
My question regards to test anti-LILRB4/Gp49 antibody A04924 on human monocyte for research purposes, then I may be interested in using anti-LILRB4/Gp49 antibody A04924 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Rodriguez
Verified customer
Asked: 2014-07-22
Answer
The products we sell, including anti-LILRB4/Gp49 antibody A04924, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-07-22